Drosophila melanogaster reference genome, annotation and cDNA (Ensembl BDGP6.32, release 106)

used by workflow runs

RNA-seq analysis workflow (HISAT2, RS2) · run 2026-08-29 12:07–22:27 CEST Workflow Run
RNA-seq analysis workflow (Hisat2, RS1) · run 2026-09-01 13:38–23:06 CEST Workflow Run
RNA-seq analysis workflow (STAR, RS1) · run 2026-08-31 09:25–17:36 CEST Workflow Run
RNA-seq analysis workflow (STAR, RS2) · run 2026-09-03 06:13–11:10 CEST Workflow Run
RNA-seq analysis workflow (Salmon, RS1) · run 2026-08-28 20:35–20:58 CEST Workflow Run
RNA-seq analysis workflow (Salmon, RS1) · run 2026-09-08 19:14–19:17 CEST Workflow Run
RNA-seq analysis workflow (Salmon, RS1) · run 2026-10-20 11:03–11:06 CEST Workflow Run
RNA-seq analysis workflow (Salmon, RS1) · run 2026-10-20 11:13–11:17 CEST Workflow Run
RNA-seq analysis workflow (Salmon, RS1) · run 2026-10-20 11:15–11:18 CEST Workflow Run
RNA-seq analysis workflow (Salmon, RS1) · run 2026-10-20 11:16–11:19 CEST Workflow Run
RNA-seq analysis workflow (Salmon, RS1) · run 2026-10-20 11:17–11:20 CEST Workflow Run
RNA-seq analysis workflow (Salmon, RS1) · run 2026-10-20 11:18–11:23 CEST Workflow Run
RNA-seq analysis workflow (Salmon, RS1) · run 2026-10-20 11:19–11:21 CEST Workflow Run
RNA-seq analysis workflow (Salmon, RS1) · run 2026-10-20 11:19–11:24 CEST Workflow Run
RNA-seq analysis workflow (Salmon, RS1) · run 2026-10-20 11:20–11:22 CEST Workflow Run
RNA-seq analysis workflow (Salmon, RS1) · run 2026-10-20 11:21–11:23 CEST Workflow Run
RNA-seq analysis workflow (Salmon, RS1) · run 2026-10-20 11:21–11:25 CEST Workflow Run
RNA-seq analysis workflow (Salmon, RS1) · run 2026-10-20 11:22–11:25 CEST Workflow Run
RNA-seq analysis workflow (Salmon, RS1) · run 2026-10-20 11:22–11:26 CEST Workflow Run
RNA-seq analysis workflow (Salmon, RS1) · run 2026-10-20 11:23–11:25 CEST Workflow Run
RNA-seq analysis workflow (Salmon, RS1) · run 2026-10-20 11:25–11:30 CEST Workflow Run
RNA-seq analysis workflow (Salmon, RS1) · run 2026-10-20 11:37–11:39 CEST Workflow Run
RNA-seq analysis workflow (Salmon, RS1) · run 2026-10-20 11:37–11:41 CEST Workflow Run
RNA-seq analysis workflow (Salmon, RS1) · run 2026-10-20 11:38–11:42 CEST Workflow Run
RNA-seq analysis workflow (Salmon, RS1) · run 2026-10-20 11:39–11:44 CEST Workflow Run
RNA-seq analysis workflow (Salmon, RS1) · run 2026-10-20 11:40–11:43 CEST Workflow Run
RNA-seq analysis workflow (Salmon, RS1) · run 2026-10-20 11:41–11:47 CEST Workflow Run
RNA-seq analysis workflow (Salmon, RS2) · run 2026-09-07 22:29–22:57 CEST Workflow Run

used by workflow

RNA-seq analysis workflow (HISAT2, RS2) Workflow
RNA-seq analysis workflow (Hisat2, RS1) Workflow
RNA-seq analysis workflow (STAR, RS1) Workflow
RNA-seq analysis workflow (STAR, RS2) Workflow
RNA-seq analysis workflow (Salmon, RS1) Workflow
RNA-seq analysis workflow (Salmon, RS2) Workflow

access and preparation instructions

https://www.ensembl.org/info/data/ftp/index.html

access statement

Assembly BDGP6.32 and Ensembl release 106 are pinned by the STAR RS2 workflow configuration.
Assembly BDGP6.32 and Ensembl release 106 are pinned by the Salmon RS2 execution configuration.
Assembly BDGP6.32 and Ensembl release 106 are pinned from CRC-FONDA/rnaseq-ninon-experiments/droso_RS1_C4_D1_salmon.config. SHA-256 checksums of the exact RUN02 inputs are included with this profile.
Assembly BDGP6.32 and Ensembl release 106 are pinned from the RS1 HISAT2 workflow configuration.
Assembly BDGP6.32 and Ensembl release 106 are pinned from the RS1 workflow configuration and were staged on the isolated STAR RS1 run volume.
Assembly BDGP6.32 and Ensembl release 106 are pinned from the RS2 workflow configuration. SHA-256 checksums of the exact RUN06 inputs are included with this profile.
Ensembl Metazoa is the upstream public source. The workflow repositories do not record which Ensembl release was used for the benchmark runs, so the exact reference version cannot currently be pinned. Recording the release number would make these runs reproducible.

input format documentation

https://www.ensembl.org/info/website/upload/gff.html

storage location

FONDA Kubernetes PVC rnaseq-pvc, mounted at /workspace/data/reference
FONDA Kubernetes PVC rnaseq-pvc, mounted read-only at /input/data/reference
FONDA Kubernetes persistent storage: /workspace/data/reference (genome FASTA, cDNA FASTA and GTF stored uncompressed)

upstream data source